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02699 A STUDY WITH THREE IMAGING TECHNIQUES OF VAGINAL GEL DISTRIBUTIONS Mauck, Christine* Background: This study evaluated factors that influence vaginal distribution of microbicidal gels, and 3 techniques for imaging and analyzing these distributions: a fiberoptic probe to detect fluorescein-labeled material, gamma scintigraphy to detect radiolabeled material, and MRI to detect gadolinium-labeled material. Methods: Replens® and K-Y® were applied in a 3.5 ml bolus in 3 parous and 3 nulligravid women at each site. Vaginal distribution was compared when the woman did not ambulate after insertion to when she did ambulate, over time intervals up to 50 min after insertion. Results: Most spreading took place in the first 20 minutes. All techniques showed better spreading of KY when women ambulated, but better spreading of Replens when they did not. MRI was best able to detect the greater linear spread of Replens than KY when women did not ambulate (p=0.06) and greater linear spreading of KY when women ambulated compared with when they did not (p=0.06). The gels are known to have different rheological properties, suggesting different vaginal distributions. Scintigraphy and MRI results were generally similar but some probe results differed since it simulated penile intromission. The degree of observed spreading was greatest with the probe due to its pushing the gel into the posterior fornix. Conclusion: Gel properties interacted with ambulation and time in influencing distributions. Each of the 3 imaging techniques provides somewhat different but complementary information about vaginal formulation distributions. The choice of which to use in research will depend on the scientific context. Christine Mauck, MD |
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