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02407 VAGINAL FLORA CHARACTERISTICS OF HIV+ WOMEN ENROLLED IN A PHASE I CELLULOSE SULFATE STUDY: HPTN 049 Justman, Jessica Background: Vaginal microbicides, if effective, may be used by HIV+ women to prevent sexual transmission of HIV. These products may affect vaginal flora.. However, there are few data on the vaginal flora characteristics of HIV+ women.. Methods: Eligibility criteria included CD4+ lymphocyte count >200cells/mm3, HIV-1 RNA <50,000 c/mm3, negative pregnancy test, normal Pap smear, no sexually transmitted infections (STI) in the preceding 6 months and no vaginal symptoms or discharge. A pelvic examination, including pH measurement and wet mount exam of vaginal fluid, was conducted prior to any microbicide use. Results: 55 HIV+ women enrolled thus far: mean age 37.9 y, 64% African American, 15% Latina, median CD4 583 cells/mm3, viral load 184 c/mm3. Antiretroviral therapy (ART) was used by 71%. Mean vaginal pH was 5.0 (range 3.5-7.0, SD 0.7), and 69% of women had a pH above normal pH of 4.5. Using clinical criteria (ph> 4.5, positive amine test and >20% clue cells), 15% of the women had bacterial vaginosis (BV). Wet mount exam did not reveal T.vaginalis or C.. albicans. Neither vaginal pH > 4.5 nor BV were associated with age, ART use, CD4 count, viral load or history of STI. Conclusions: These asymptomatic, highly selected HIV-infected women had a high prevalence of elevated vaginal pH and incidental BV at baseline. As BV may act as a co-factor in the heterosexual transmission of HIV, the impact of vaginal microbicides on BV warrants further study. Jessica Justman, MD |
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