Microbicides 2004 Microbicides 200428-31 March 2004, Hilton London MetropoleThe conference42 million men, women and children worldwide were living with HIV by the end of December 2002 (source: UNAIDS), including five million newly-infected during that year alone. Another 45 million people will become infected between 2002 and 2010, unless the current transmission rates can be vastly reduced. Of the 42 million, 29.4 million live in sub-Saharan Africa and 58% of them are women. Not only are women more susceptible to HIV infection, many are powerless to insist on the use of condoms or other methods of protecting themselves. In this context, and with the knowledge that an effective HIV vaccine is unlikely to be available for several years, the need for an effective topical microbicide grows ever more urgent. 2004 should prove to be a landmark year in the field of microbicide development as the first Phase III trials of novel products are due to start – the next step along the road to making a microbicide available to the millions worldwide in desperate need of protection.The aims of the Microbicides 2004 conference are to:Report novel or innovative work in the microbicides fieldProvide updates on recent microbicides research, divided into three tracks: basic science, clinical science, and behavioural science (including public health and the microbicide marketplace)Provide a forum for the discussion of new developments in microbicide research including ethical, clinical, behavioural and methodological issuesPresent opportunities for knowledge-sharing between microbicide researchers, public-health workers and advocacy organisations.There will be an opening ceremony on the evening of Sunday 28 March at which politicians, policy makers and the international media are expected. The conference will run for a full three days, each of which will contain:Scientific overviews and presentations with plenary sessions, invited lecturers and presentations of original researchWorkshops to review issues unique to microbicides such as trial design and outcome measures, and ethical issues in the clinical trials of microbicidesPoster sessions. Focus on LondonFollowing the successful Microbicides conferences in Washington in 2000 and Antwerp in 2002, March 2004 sees the focus move to London.The venue is the Hilton Metropole Hotel, two minutes by taxi from Paddington station and the Heathrow Express, with a journey time from the airport of 15 minutes. The hotel is in walking distance of Hyde Park and London’s main shopping streets, and close to Imperial College. Accommodation will be available at the venue and other hotels in the vicinity.London in March offers a variety of diversions for out-of-conference relaxation, including sight-seeing and shopping; the arts and the theatre; and pubs, clubs and restaurants to suit every taste. Conference staff will be on hand to help delegates plan their spare time.To book your place or find out more information, e-mail info@microbicides2004.org.uk or telephone the Event Office on +44 (0) 20 7720 4411
Oral: invited speaker Oral: Track A Oral: Track B Oral: Track C Poster: Track A Poster: Track B Poster: Track C Abstract only Authors

02595 CONTRACEPTIVE ACTION OF CELLULOSE ACETATE PHTHALATE (CAP)

Morshedi, Mahmood1
Lattanzio, F2, Swanson, RJ3, Mahony, M4 and Neurath, R5
1, 2Eastern Virginia Medical School, Norfolk, VA, 3Old Dominion University, Norfolk, VA, 4Organon Pharmaceutical, New Jersey, NJ and 5the New York Blood Center, NY

We previously reported that CAP containing formulations 803-39 and 803-40 at 0.6 gram of formulation/mL semen were able to render human sperm completely immotile, non viable and acrosome reacted. At 0.3 gram/mL, they were also 100% effective in preventing sperm penetration into bovine cervical mucus. Here, we report the preliminary results of our ongoing investigations on the contraceptive action of CAP using a rabbit model. Additionally, we are utilizing electron and confocal microscopy as well as calcium influx to further investigate the nature of CAP impact on sperm. New Zealand albino rabbits were either inseminated using pre-treated sperm (CAP at 0.3-0.6 gram/mL semen) or allowed to mate following intravaginal placement of CAP gel at similar concentrations. No rabbits achieved pregnancies following inseminations with pre-treated sperm. However, intravaginal application of the compounds followed by mating of the does resulted in less than marginal effectiveness (20%, n=5 for each experiment). Multiple mating, dilution with vaginal secretions and difficulty in assessing the volume of semen following natural mating (to adjust for the concentration of intravaginal CAP) were contributory to the marginal in vivo contraceptive performance of CAP. Remedies are currently being implemented/investigated. Irritation of the vaginal epithelium in the rabbits studied was non-significant. Compared to controls and CAP vehicles, CAP formulations induced a significant (p<0.003) increase in calcium influx at 0.3 gram formulation/mL semen. Results of complete evaluations (i.e., histological) along with the confocal and electron microscopy studies assessing the impact of CAP on sperm will be presented.

Dr. Mahmood Morshedi
EVMS, 601 Colley Avenue, Norfolk, VA 23507, USA
(Telephone)  757-446-5029 (Fax)  757-446-5052 (E-mail)  morshems@evms.edu