Microbicides 2004 Microbicides 200428-31 March 2004, Hilton London MetropoleThe conference42 million men, women and children worldwide were living with HIV by the end of December 2002 (source: UNAIDS), including five million newly-infected during that year alone. Another 45 million people will become infected between 2002 and 2010, unless the current transmission rates can be vastly reduced. Of the 42 million, 29.4 million live in sub-Saharan Africa and 58% of them are women. Not only are women more susceptible to HIV infection, many are powerless to insist on the use of condoms or other methods of protecting themselves. In this context, and with the knowledge that an effective HIV vaccine is unlikely to be available for several years, the need for an effective topical microbicide grows ever more urgent. 2004 should prove to be a landmark year in the field of microbicide development as the first Phase III trials of novel products are due to start – the next step along the road to making a microbicide available to the millions worldwide in desperate need of protection.The aims of the Microbicides 2004 conference are to:Report novel or innovative work in the microbicides fieldProvide updates on recent microbicides research, divided into three tracks: basic science, clinical science, and behavioural science (including public health and the microbicide marketplace)Provide a forum for the discussion of new developments in microbicide research including ethical, clinical, behavioural and methodological issuesPresent opportunities for knowledge-sharing between microbicide researchers, public-health workers and advocacy organisations.There will be an opening ceremony on the evening of Sunday 28 March at which politicians, policy makers and the international media are expected. The conference will run for a full three days, each of which will contain:Scientific overviews and presentations with plenary sessions, invited lecturers and presentations of original researchWorkshops to review issues unique to microbicides such as trial design and outcome measures, and ethical issues in the clinical trials of microbicidesPoster sessions. Focus on LondonFollowing the successful Microbicides conferences in Washington in 2000 and Antwerp in 2002, March 2004 sees the focus move to London.The venue is the Hilton Metropole Hotel, two minutes by taxi from Paddington station and the Heathrow Express, with a journey time from the airport of 15 minutes. The hotel is in walking distance of Hyde Park and London’s main shopping streets, and close to Imperial College. Accommodation will be available at the venue and other hotels in the vicinity.London in March offers a variety of diversions for out-of-conference relaxation, including sight-seeing and shopping; the arts and the theatre; and pubs, clubs and restaurants to suit every taste. Conference staff will be on hand to help delegates plan their spare time.To book your place or find out more information, e-mail info@microbicides2004.org.uk or telephone the Event Office on +44 (0) 20 7720 4411
Oral: invited speaker Oral: Track A Oral: Track B Oral: Track C Poster: Track A Poster: Track B Poster: Track C Abstract only Authors

02466 OPTIMIZING EXPRESSION OF PROTEIN MICROBICIDES IN LACTOBACILLI: BENEFITS OF GENOMIC SEQUENCING

Chang, Chia-Hwa
Cheng, J.*, Frankel, K.*, Essenmacher, K., Simpson, D., Lagenaur L., Parks, T., Martin, P., Liu, X., Xu, Q., and Lewicki, J.
Osel, Inc., 1800 Wyatt Dr., Suite 14, Sanata Clara, CA 95054; *Genomics Division, Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720

Osel is actively pursuing genetic modification of native lactobacilli as a vehicle to deliver protein microbicides to vaginal mucosal membranes as a novel means of impeding the transmission of HIV and STDs. The success of this approach, termed MucoCept, largely depends on whether human vaginal isolates of lactobacilli are amenable to genetic manipulation thereby enabling expression of active virus-binding proteins to intercept infectious viruses at the mucosal surface.

To support the facile genetic manipulation and overcome inefficient heterologous expression in vaginal lactobacilli, the genomic sequence of Lactobacillus jensenii 1153 was determined. Detailed reporter-based analyses of the genomic data containing over 1600 ORFs have identified novel cell wall anchor domains, unique signal sequences, powerful promoter elements, and possible sites for chromosomal integration of heterologous genes. These proprietary native sequences have been used to direct more efficient expression of heterologous proteins, including those anchored covalently to the cell wall of L. jensenii, such as CD4 and cyanovirin-N. Some of these sequences have also been employed to stabilize heterologous fusion proteins. Furthermore, novel promoter elements have enabled expression of these proteins over a 50-fold range of concentrations. It is anticipated that all of these native regulatory components will be used in our final MucoCept HIV product format.

Chia-Hwa, Chang, Senior Scientist
Osel Inc., 1800 Wyatt Dr., Suite 14, Santa Clara, CA 95054, USA
(Telephone) (408)-986-0012 ext 202 (Fax) (408)-986-0019 (E-mail) cchang@oselinc.com