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02342 ESTABLISHING A CHLAMYDIA + SHIV CO-INFECTION MODEL IN NONHUMAN PRIMATES: PRELIMINARY DATA Patton, Dorothy Prevention of human immunodeficiency virus (HIV) remains a primary goal in Topical Microbicide product development. Preclinical studies designed to test a products ability to prevent infection in the face of viral challenge are essential for product advancement to clinical trials. Conducting such studies in nonhuman primates (NHP) may provide results most apt to translate to the human experience. We have initiated model development studies to establish a reliable SHIV infection model in Macaca nemestrina monkeys. In this model, animals are pre-exposed to infection with Chlamydia trachomatis (CT). With a heightened anti-CT immune response, animals undergo a single mucosal challenge (intravaginal) with SHIV, chimeric SIV/ HIV which has been molecularly constructed for macaque infection. The initial series of six-animal experiments are completed in which 3 animals were pre-exposed to chlamydia cervical infection prior to mucosal challenge with 500 TCID50 SHIV89.6P, while the remaining three underwent SHIV inoculation without prior CT exposure. All three of the pre-exposed animals became infected with SHIV, while one of three SHIV-only animals has developed viral infection, as of week 10 post-SHIV challenge. These results indicate that CT pre-exposure may have enhanced SHIV transmission and/or infection, and constitute the basis for a new, CT/SHIV co-infection model in non-human primates. This work supported by CONRAD MSA-02-315 and U of WA National Primate Research Center RR-00166. Dr. Dorothy Patton |
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